Platform for Bioassays for screening antitumor compounds

Fiocruz’s Network Technological Platforms
Antitumoral screening facility offer cell-based assays for determination of antitumoral activity of small molecules, biologicals, natural products, and nanostructured products. Using human tumoral cell lines derived from several organs and tissues provide support for compound isolation- and synthesis-guided by bioactivity, selectivity determination, IC50 and assays for apoptosis and caspase 3/7 activation. Cytotoxicity assays are based on MTT method, and annexin V/PI and caspase 3/7 activity performed by flow cytometry. Assays using 3D spheroid method are available on demand.

This platform is located in FIOCRUZ – Rio de Janeiro (FAR).